產(chǎn)品英文名稱(chēng)：RoboSep™ Human Whole Blood CD34 Positive Selection Kit with Filter Tips
產(chǎn)品中文名稱(chēng)：RoboSep™ 帶有過(guò)濾嘴的人血CD34陽(yáng)性選擇試劑盒

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RoboSep™ Human Whole Blood CD34 Positive Selection Kit with Filter Tips產(chǎn)品英文描述
RoboSep™ Human Whole Blood CD34 Positive Selection Kit with Filter Tips is designed to isolate CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells. If isolating CD34+ cells from fresh cord blod, we recommend using the EasySep™ Human Cord Blood CD34 Positive Selection Kit (Catalog #18096). If isolating CD34+ cells from any other sample type, we recommend using the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056).

RoboSep™ 帶有過(guò)濾嘴的人血CD34陽(yáng)性選擇試劑盒產(chǎn)品組分
? EasySep™ Human Whole Blood CD34 Positive Selection Cocktail, 3 x 1.0 mL
? EasySep™ Magnetic Particles, 3 x 1.0 mL
? EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL

RoboSep™ Human Whole Blood CD34 Positive Selection Kit with Filter Tips *使用
The isolation of human CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection

RoboSep™ 帶有過(guò)濾嘴的人血CD34陽(yáng)性選擇試劑盒保存方法
EasySep™ Human Whole Blood CD34 Positive Selection Cocktail
Stable at 4°C for 2 years. Do not freeze this product. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep™ Magnetic Nanoparticles EasySep
Stable at 4°C for 2 years. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep™ RBC Lysis Buffer 10X Concentrate EasySep
10X concentrate is stable at room temperature for 2 years. 1X lysis buffer is stable at 4°C for 3 months. Do not freeze.

RoboSep™ Human Whole Blood CD34 Positive Selection Kit with Filter Tips 實(shí)驗(yàn)資料
FACS Profile Results with EasySep® Human Buffy Coat/Whole Blood CD34 Positive Selection Kit

Starting with buffy coat, the CD34+ cell content of the enriched fraction typically ranges from 51.7% - 90.3% (reported as a percentage of viable CD45+ cells).

Typical EasySep™ Procedure for Human Cells

RoboSep™ 帶有過(guò)濾嘴的人血CD34陽(yáng)性選擇試劑盒相關(guān)產(chǎn)品訂購(gòu)信息
Stemcell  10513  CD34 Antibody, Clone 8G12, PE-Conjugated  Mouse Monoclonal Antibody to Human CD34 - PE Conjugated 
Stemcell  10413  CD34 Antibody, Clone 8G12, FITC-Conjugated  Mouse Monoclonal Antibody to Human CD34 - FITC Conjugated 
Stemcell  10613  CD34 Antibody, Clone 8G12, APC-Conjugated  Mouse Monoclonal Antibody to Human CD34 - PE Conjugated 
Stemcell  07801  Lymphoprep™  Density Gradient Medium for the Isolation of Mononuclear Cells 
Stemcell  20110  EasySep™ Red Blood Cell Lysis Buffer  Red Blood Cell Lysis Buffer 
Stemcell  07800  Ammonium Chloride Solution  Reagent for the Lysis of Red Blood Cells 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  04034  MethoCult™ H4034 Optimum  Methylcellulose-Based Medium with Recombinant Cytokines and EPO for Human Cells 
Stemcell  04435  MethoCult™ H4435 Enriched  Methylcellulose Medium with Recombinant Cytokines and EPO for Human Cells 
Stemcell  04434  MethoCult™ H4434 Classic  Methylcellulose-Based Medium with Recombinant Cytokines for Human Cells
Stemcell  60013  Anti-Human CD34 Antibody, Clone 581  Mouse Monoclonal Antibody Against Human CD34          
For research use only. Not intended for human or animal diagnostic or therapeutic uses.

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StemRD   MGro-500  MSC medium,chemically-defined,間充質(zhì)干細(xì)胞培養(yǎng)基 500mL/瓶

LONZA   04-418Q    X-VIVO 15 無(wú)血清培養(yǎng)基 1L/瓶

Life Technology   087-0112DK    AIM-V® CTS™ 無(wú)血清細(xì)胞培養(yǎng)基（細(xì)胞治療級(jí)） 1000mL/瓶

Pall   15950-017  Ultroser Serum Substitute 血清替代品 20mL/瓶

LONZA   12-725F   UltraCULTURE Serum-free Medium無(wú)血清培養(yǎng)基 500mL/瓶

BD 762165   PAXgene Blood RNA Tube RNA樣本收集管 100管/盒

LONZA 50150   SeaKem® Gold Agarose 金瓊脂糖粉 125g/瓶

LONZA CC-3162   EGM-2 BulletKit( CC-3156 & CC-4176 )套裝 Kit/套

Nunc 159910   175cm2易用培養(yǎng)瓶,PS,已滅菌,瓶蓋:過(guò)濾蓋 30個(gè)/箱

LONZA LT07-318  MycoAlert® Mycoplasma Detection Kit支原體檢測(cè)試劑盒 100Tests/盒

LONZA   LT07-418 MycoAlert® Mycoplasma Detection Kit支原體檢測(cè)試劑盒 50Tests/盒

Lonza   50-650U   Kinetic-QCL 192 Test Kit內(nèi)毒素檢測(cè)試劑盒   192T/盒

Corning   431080    175cm⒉細(xì)胞培養(yǎng)瓶，Angled頸，透氣蓋 50個(gè)/箱

Corning   8388  125mL儲(chǔ)液瓶 Storage Bottles,PS,45mm瓶頸，帶蓋，滅菌，獨(dú)立包裝 24個(gè)/箱

Corning   430659    2ml凍存管, 聚丙烯，外旋蓋, 可自立圓底 500個(gè)/箱

Corning   3290     75cm2 CellBIND Surface培養(yǎng)瓶，已滅菌，透氣蓋 100個(gè)/箱

Corning   3292     175cm2 CellBIND Surface培養(yǎng)瓶，已滅菌，透氣蓋 50個(gè)/箱

BD-Falcon   352360   Cell Strainer, 100µm, Yellow 100um細(xì)胞濾網(wǎng) 50個(gè)/箱

BD Falcon 352070 50mL錐形離心管，高透明度聚丙烯,袋裝 500支/箱

BD Falcon 352096 15mL錐形離心管，高透明度聚丙烯,袋裝 500支/箱

BD BioCoat 354234   Matrigel Basement Membrane Matrix(標(biāo)準(zhǔn)型) 10mL/瓶

Cygnus   F550   CHO HCP ELISA Kit,3G宿主蛋白檢測(cè)試劑盒 96T/盒

Cygnus   F410   E.coli HCP ELISA Kit 大腸桿菌 96T/盒

Nalgene  5100-0001 Cryo 1℃ Freezing Container梯度降溫凍存盒 1個(gè)/箱

Nalgene    5026-0909   CryoBoxes 凍存盒 9X9 陣列，管瓶1.2/2.0mL   24個(gè)/箱

Nalgene   2250-0130 50L 可高溫高壓操作的細(xì)口大瓶（帶手柄）   1個(gè)/箱

Nalgene   2319-0050    20L 可高溫高壓滅菌的細(xì)口大瓶（帶放水口）    1個(gè)/箱

Hyclone  SH30084.03 FBS 優(yōu)等澳洲胎牛血清 500mL/瓶

PeproTech   100-18B-500   Recombinant Human FGF-basic (154 a.a.)因子 500ug/支

Parafilm PM-996   Parafilm® M Laboratory Film 封口膜 12卷/箱